Study Confirms approximately 50% of stem cells DIE in culture within one week AND osteoarthritic cartilage has a deficiency of stem cells
Can we stimulate articular cartilage growth in arthritis? While researchers look for the “magic factor” to stimulate articular cartilage repair, it is surely clear from anyone familiar with osteoarthritis research that the condition is multi factorial. Some of the factors related to development of osteoarthritis involve joint instability, ligament laxity, growth factors, and of course stem cells.
I recently reviewed a study that appeared in Arthritis Research Therapeutics from The Scripps Research Institute that confirmed that osteoarthritic cartilage has a deficiency of stem cells.1 The researchers took articular cartilage from 40 human donors. Sixteen of the articular cartilage samples were normal and 23 of the donors had osteoarthritis. The researchers found the osteoarthritic cartilage contained 25% fewer stem cells than normal cartilage. Though for the CD 166 marker (a marker for stem cells) osteoarthritic cartilage stem cells were reduced by over 80%. They also found that about 50% of the cells in cartilage possess markers that signify that they are stem cells.
Stem cell counts decline while being cultured: What most intrigued me about this study was the fact that when stem cell counts were taken, an over 50% drop in numbers was observed after one week in culture. Quoting the authors, “Stro-1 levels in cells cultured for only 24 hours were 24.6%, but this dropped to below 10% by 7 days.”1
Again to those researching stem cell injection therapy treatments, I will again quote the author, “The number of starting stem cells is not the issue. The issue is how many survive over the course of the six months of regeneration.” This study, as well as others, confirms that most cultured stem cells do not survive.
This is one of the many reasons Caring Medical uses direct stem cell therapy which means we inject them immediately after obtaining them. We extract the patient’s bone marrow from the iliac crest (pelvis) or tibia (shin) and then inject it immediately (without culturing) into the degenerated area. In other words, there is minimal amount of time outside of the body, thus all the injected stem cells and bone marrow cells are of optimum health because they have not been manipulated. We don’t change them! Why? We believe the body knows best! Let the body change the cells into whatever cells it needs. The degenerated joint needs a lot more than cartilage cells, it needs also meniscus cells, synovial cells, as well as a lot of other factors that go into growing extracellular matrix. This is why our stem cell therapy is Bone Marrow Prolotherapy.
For more information: www.bonemarrowprolotherapy.com
For appointment information:contact us.
1. Grogan SP, Miyaki S., Asahara H. Mesenchymal progenitor cell markers in human articular cartilage: normal distribution and changes in osteoarthritis. Arthritis Research Therapeutics. 2009: 11(4):121.